Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/34499
Type of publication: Straipsnis / Article
Author(s): Šatkauskas, Saulius;Kaselis, Andrius;Treinys, Rimantas;Bagnard, Dominique;Jover, Emmanuel
Title: R-type calcium channels are crucial for Semaphorin 3A-induced DRG axon growth cone collapse
Is part of: Plos one, 2014, Vol. 9, no. 7, p. 1-14
Date: 2014
Keywords: Semaforinai;Kalcio kanalai;Aksonai;Semaphorins;Calcium channels;Axons
Abstract: Semaphorin 3A (Sema3A) is a secreted protein involved in axon path-finding during nervous system development. Calcium signaling plays an important role during axonal growth in response to different guidance cues; however it remains unclear whether this is also the case for Sema3A. In this study we used intracellular calcium imaging to figure out whether Sema3Ainduced growth cone collapse is a Ca2+ dependent process. Intracellular Ca2+ imaging results using Fura-2 AM showed Ca2+ increase in E15 mice dorsal root ganglia neurons upon Sema3A treatment. Consequently we analyzed Sema3A effect on growth cones after blocking or modifying intracellular and extracellular Ca2+ channels that are expressed in E15 mouse embryos. Our results demonstrate that Sema3A increased growth cone collapse rate is blocked by the non-selective R- and T- type Ca2+ channel blocker NiCl2 and by the selective R-type Ca2+ channel blocker SNX482. These Ca2+ channel blockers consistently decreased the Sema3A-induced intracellular Ca2+ concentration elevation. Overall, our results demonstrate that Sema3A-induced growth cone collapses are intimately related with increase in intracellular calcium concentration mediated by R-type calcium channels.
Internet: https://eltalpykla.vdu.lt/1/34499
http://dx.doi.org/10.1371/journal.pone.0102357
https://hdl.handle.net/20.500.12259/34499
Appears in Collections:1. Straipsniai / Articles

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