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Type of publication: conference paper
Type of publication (PDB): Tezės Clarivate Analytics Web of Science / Theses in Clarivate Analytics Web of Science (T1a)
Field of Science: Medicina / Medicine (M001);Biologija / Biology (N010)
Author(s): Streleckienė, Greta;Inčiūraitė, Rūta;Juzėnas, Simonas;Šaltenienė, Violeta;Steponaitienė, Rūta;Gyvytė, Ugnė;Ruzgys, Paulius;Link, Alexander;Kupčinskas, Juozas;Skiecevičienė, Jurgita
Title: miR-20b and miR-451a are involved in gastric carcinogenesis through the PI3K/AKT/mTOR signaling pathway: data from gastric cancer patients, cell lines and INS-GAS mouse model
Is part of: European journal of human genetics. London : Springer Nature, 2020, Vol. 28, suppl. 1
Extent: p. 534-535
Date: 2020
Note: Abstracts from the 53rd European Society of Human Genetics (ESHG) Conference : interactive e-Posters : virtual conference, June 6-9, 2020, Berlin, Germany
Abstract: Introduction: Gastric cancer (GC) is one of the most prevalent and lethal cancer worldwide. Many studies have shown that development of malignancies is mainly driven by alterations of cellular signaling pathways. MicroRNAs are small noncoding molecules playing an essential role in various biological processes. The aim of this study was to evaluate the functional role of miR-20b and miR-451a and their target genes in GC. Materials and methods: To determine the expression level of miR-20b and miR-451a in vivo and in vitro, biopsy samples of GC patients, healthy controls, INS-GAS mice, AGS and MKN28 cell lines samples were analysed using qRT-PCR. To confirm putative microRNA target-genes and determine miR-20b and miR-451a impact on cell function, luciferase reporter, protein expression, MTT, clonogenic, apoptosis, wound healing assays were performed in GC cell cultures. Results: Deregulated expression of miR-20b and miR-451a was determined in GC cell lines and the INS-GAS mouse model. Western blot and luciferase reporter assay have also confirmed that miR-20b directly regulates expression of PTEN and TXNIP, and miR-451a - CAV1 and TSC1. Loss-of-function experiments revealed that down-regulation of miR-20b and upregulation of miR-451a expression exhibits an anti-tumor effect in vitro (miR-20b: reduced viability, colony formation, increased apoptosis rate, and miR-451a: reduced colony forming ability)
Affiliation(s): Biologijos katedra
Lietuvos sveikatos mokslų universitetas. Medicinos akademija
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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