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Type of publication: research article
Type of publication (PDB): Straipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)
Field of Science: Biologija / Biology (N010);Medicina / Medicine (M001)
Author(s): Streleckienė, Greta;Inčiūraitė, Rūta;Juzėnas, Simonas;Šaltenienė, Violeta;Steponaitienė, Rūta;Gyvytė, Ugnė;Kiudelis, Gediminas;Leja, Marcis;Ruzgys, Paulius;Šatkauskas, Saulius;Kupčinskienė, Eugenija;Franke, Sabina;Thon, Cosima;Link, Alexander;Kupčinskas, Juozas;Skiecevičienė, Jurgita
Title: miR-20b and miR-451a are involved in gastric carcinogenesis through the PI3K/AKT/mTOR signaling pathway: data from gastric cancer patients, cell lines and Ins-Gas mouse model
Is part of: International journal of molecular sciences. Basel : MDPI AG, 2020, vol. 21, iss. 3
Extent: p. 1-16
Date: 2020
Note: Article no. 877
Keywords: microRNAs;miR-20b;miR-451a;PI3K/AKT/mTOR signaling pathway;Gastric cancer
Abstract: Gastric cancer (GC) is one of the most common and lethal gastrointestinal malignancies worldwide. Many studies have shown that development of GC and other malignancies is mainly driven by alterations of cellular signaling pathways. MicroRNAs (miRNAs) are small noncoding molecules that function as tumor-suppressors or oncogenes, playing an essential role in a variety of fundamental biological processes. In order to understand the functional relevance of miRNA dysregulation, studies analyzing their target genes are of major importance. Here, we chose to analyze two miRNAs, miR-20b and miR-451a, shown to be deregulated in many di erent malignancies, including GC. Deregulated expression of miR-20b and miR-451a was determined in GC cell lines and the INS-GAS mouse model. UsingWestern Blot and luciferase reporter assay we determined that miR-20b directly regulates expression of PTEN and TXNIP, and miR-451a: CAV1 and TSC1. Loss-of-function experiments revealed that down-regulation of miR-20b and up-regulation of miR-451a expression exhibits an anti-tumor e ect in vitro (miR-20b: reduced viability, colony formation, increased apoptosis rate, and miR-451a: reduced colony forming ability). To summarize, the present study identified that expression of miR-20b and miR-451a are deregulated in vitro and in vivo and have a tumor suppressive role in GC through regulation of the PI3K/AKT/mTOR signaling pathway
Affiliation(s): Biologijos katedra
Lietuvos sveikatos mokslų universitetas. Medicinos akademija
Vytauto Didžiojo universitetas
Appears in Collections:1. Straipsniai / Articles
Universiteto mokslo publikacijos / University Research Publications

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